Term Papers 4 pages, words To investigate the effect of temperature on the enzyme Catalase. To investigate the effect of temperature on the enzyme Catalase. We will conclude the experiment by establishing the enzymes optimum temperature the temperature at which it works best at. We will also carry out some simple experiments using the substrate Hydrogen Peroxide.
Investigating an enzyme-controlled reaction: Hydrogen peroxide is harmful and must be removed as soon as it is produced in the cell.
Cells make the enzyme catalase to remove hydrogen peroxide.
This investigation looks at the rate of oxygen production by the catalase in pureed potato as the concentration of hydrogen peroxide varies.
The oxygen produced in 30 seconds is collected over water. Then the rate of reaction is calculated. Lesson organisation You could run this investigation as a demonstration at two different concentrations, or with groups of students each working with a different concentration of hydrogen peroxide.
Individual students may then have time to gather repeat data. Groups of three could work to collect results for 5 different concentrations and rotate the roles of apparatus manipulator, result reader and scribe.
Collating and comparing class results allows students to look for anomalous and inconsistent data. Apparatus and Chemicals For each group of students: Wash splashes of pureed potato or peroxide off the skin immediately. Be aware of pressure building up if reaction vessels become blocked. Take care inserting the bung in the conical flask — it needs to be a tight fit, so push and twist the bung in with care.
Take care when removing the cap of the reagent bottle, as gas pressure may have built up inside. Dilute immediately before use and put in a clean brown bottle, because dilution also dilutes the decomposition inhibitor. Keep in brown bottles because hydrogen peroxide degrades faster in the light.
Discard all unused solution. Do not return solution to stock bottles, because contaminants may cause decomposition and the stock bottle may explode after a time.
You might need to add water to make it less viscous and easier to use. Discs of potato react too slowly. Wear eye protection and protect clothing from hydrogen peroxide.
Rinse splashes of peroxide and pureed potato off the skin as quickly as possible.Apr 30, · This is because as there are progressively fewer molecules of hydrogen peroxide there will be fewer collisions between the substrate and enzyme molecules (catalase in yeast), leading to a decrease in enzyme-substrate complexes alphabetnyc.coms: 5.
Conclusion Activity A: Hypothesis: If hydrogen peroxide is introduced to catalase, then the rate at which the decomposition of by catalase will increase because enzymes are proteins that catalyze chemical reactions. Decomposition of hydrogen peroxide is a chemical reaction, therefore, its rate will increase because, after all, catalase is an enzyme.
Catalase breaks down the hydrogen peroxide into water and oxygen before it damages the cell (Frederick, ).
The purpose of our lab is to prove  how catalase speeds up the break down of hydrogen peroxide and determine the reaction rate at which it does . Biology Enzymes Coursework Prediction: I think that the enzyme will work best at c to c I think this because that optimum temperature for most natural enzymes is c but his is a chemical enzyme so it will work best a little higher.
Apr 30, · Here is an example of A-level biology coursework on the effect of substrate concentration (hydrogen peroxide) on the rate of activity of the enzyme alphabetnyc.coms: 5. Hydrogen peroxide (H 2 O 2) is a by-product of respiration and is made in all living cells.
Hydrogen peroxide is harmful and must be removed as soon as it is produced in the cell. Cells make the enzyme catalase to remove hydrogen peroxide.